overlay medium dmem containing methylcellulose 1 Search Results


methylcellulose erythropoietin  (Kirin Brewery Company)
90
Kirin Brewery Company methylcellulose erythropoietin
Methylcellulose Erythropoietin, supplied by Kirin Brewery Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methylcellulose  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc methylcellulose
Methylcellulose, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isopto alkaline  (alcon inc)
90
alcon inc isopto alkaline
Isopto Alkaline, supplied by alcon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methocult gf3434  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc methocult gf3434
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methylcellulose sf h4436 medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc methylcellulose sf h4436 medium
Methylcellulose Sf H4436 Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methylcellulose stem cell  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc methylcellulose stem cell
Methylcellulose Stem Cell, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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semisolid methylcellulose medium  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc semisolid methylcellulose medium
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polyvinyl acetates and derivatives kollicoat  (BASF)
90
BASF polyvinyl acetates and derivatives kollicoat
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methylcellulose 4000  (FUJIFILM)
90
FUJIFILM methylcellulose 4000
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methylcellulose  (R&D Systems)
95
R&D Systems methylcellulose
FIG. 1. Transformation of CD9-selected testis cells in vitro. A) TUNEL staining of the M-transfected GS or CD9-selected testis cells. B) Quantification of cells that underwent apoptosis. At least 2500 cells were counted in two different experiments. C) Morphological appearance of CD9-selected cells that were transduced by the indicated genes. Stable colonies were found when DMR or KMSO genes were transfected. Although transfection of MR or DM genes also produced similar colonies, they disappeared after one to two passages. The pictures were taken between 6 and 8 days after transfection. D) Colonies of DMR- or KMSO-transfected cells in <t>methylcellulose</t> 1 wk after plating. E) Alkaline phosphatase staining. F) Comparison of the frequency of colony development. Aliquots of 13105 cells were plated in a 12-well plate and transduced with DMR or KMSO genes in two experiments (n¼20). Bar¼ 100 lm (A), 250 lm (C, E), and 25 lm (D).
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murine methocult medium m3231  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc murine methocult medium m3231
FIG. 1. Transformation of CD9-selected testis cells in vitro. A) TUNEL staining of the M-transfected GS or CD9-selected testis cells. B) Quantification of cells that underwent apoptosis. At least 2500 cells were counted in two different experiments. C) Morphological appearance of CD9-selected cells that were transduced by the indicated genes. Stable colonies were found when DMR or KMSO genes were transfected. Although transfection of MR or DM genes also produced similar colonies, they disappeared after one to two passages. The pictures were taken between 6 and 8 days after transfection. D) Colonies of DMR- or KMSO-transfected cells in <t>methylcellulose</t> 1 wk after plating. E) Alkaline phosphatase staining. F) Comparison of the frequency of colony development. Aliquots of 13105 cells were plated in a 12-well plate and transduced with DMR or KMSO genes in two experiments (n¼20). Bar¼ 100 lm (A), 250 lm (C, E), and 25 lm (D).
Murine Methocult Medium M3231, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methylcellulose based medium  (Miltenyi Biotec)
93
Miltenyi Biotec methylcellulose based medium
FIG. 1. Transformation of CD9-selected testis cells in vitro. A) TUNEL staining of the M-transfected GS or CD9-selected testis cells. B) Quantification of cells that underwent apoptosis. At least 2500 cells were counted in two different experiments. C) Morphological appearance of CD9-selected cells that were transduced by the indicated genes. Stable colonies were found when DMR or KMSO genes were transfected. Although transfection of MR or DM genes also produced similar colonies, they disappeared after one to two passages. The pictures were taken between 6 and 8 days after transfection. D) Colonies of DMR- or KMSO-transfected cells in <t>methylcellulose</t> 1 wk after plating. E) Alkaline phosphatase staining. F) Comparison of the frequency of colony development. Aliquots of 13105 cells were plated in a 12-well plate and transduced with DMR or KMSO genes in two experiments (n¼20). Bar¼ 100 lm (A), 250 lm (C, E), and 25 lm (D).
Methylcellulose Based Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 1. Transformation of CD9-selected testis cells in vitro. A) TUNEL staining of the M-transfected GS or CD9-selected testis cells. B) Quantification of cells that underwent apoptosis. At least 2500 cells were counted in two different experiments. C) Morphological appearance of CD9-selected cells that were transduced by the indicated genes. Stable colonies were found when DMR or KMSO genes were transfected. Although transfection of MR or DM genes also produced similar colonies, they disappeared after one to two passages. The pictures were taken between 6 and 8 days after transfection. D) Colonies of DMR- or KMSO-transfected cells in methylcellulose 1 wk after plating. E) Alkaline phosphatase staining. F) Comparison of the frequency of colony development. Aliquots of 13105 cells were plated in a 12-well plate and transduced with DMR or KMSO genes in two experiments (n¼20). Bar¼ 100 lm (A), 250 lm (C, E), and 25 lm (D).

Journal: Biology of reproduction

Article Title: In vitro transformation of mouse testis cells by oncogene transfection.

doi: 10.1095/biolreprod.111.095307

Figure Lengend Snippet: FIG. 1. Transformation of CD9-selected testis cells in vitro. A) TUNEL staining of the M-transfected GS or CD9-selected testis cells. B) Quantification of cells that underwent apoptosis. At least 2500 cells were counted in two different experiments. C) Morphological appearance of CD9-selected cells that were transduced by the indicated genes. Stable colonies were found when DMR or KMSO genes were transfected. Although transfection of MR or DM genes also produced similar colonies, they disappeared after one to two passages. The pictures were taken between 6 and 8 days after transfection. D) Colonies of DMR- or KMSO-transfected cells in methylcellulose 1 wk after plating. E) Alkaline phosphatase staining. F) Comparison of the frequency of colony development. Aliquots of 13105 cells were plated in a 12-well plate and transduced with DMR or KMSO genes in two experiments (n¼20). Bar¼ 100 lm (A), 250 lm (C, E), and 25 lm (D).

Article Snippet: Anchorage independent growth of transformed cells was examined by suspending 2 3 105 cells in 1 ml of methylcellulose (R&D Systems, Minneapolis, MN), followed by culture in a 24-well plate.

Techniques: Transformation Assay, In Vitro, TUNEL Assay, Staining, Transfection, Produced, Comparison, Transduction